Information

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The SYL175 Scyliorhinus canicula qPCR Detection kit has been developed for accurate and consistent environmental DNA (eDNA) detection. Together with the Sylphium eDNA qPCR Mix, this kit delivers exceptional sensitivity, robustness, and accuracy in qPCR-based detection assays. The eDNA qPCR Mix is highly resistant to inhibitory substances, such as humic acids present in environmental samples.

This kit contains primers and a probe designed to detect a highly specific region of the mitochondrial DNA of Scyliorhinus canicula.


Detection as a service:

We offer the detection of Scyliorhinus canicula as a service. Visit the analysis page or contact us directly for more information about sampling, shipping, and pricing.


Kit contents:

• Primer/probe mix (10x)
  
• eDNA qPCR Mix, containing:
  
  • eTaq DNA polymerase
    
  • eTaq qPCR mix
    
  • PCR-grade water
    
• Positive control

Sufficient for 200 reactions of 25 ul each.


Common species names:

• English: Small-spotted catshark
  
• German: Kleingefleckter Katzenhai
  
• French: Petite roussette
  
• Dutch: Hondshaai
  

All kits are compatible with major qPCR platforms, including Bio-Rad, QuantStudio, and Applied Biosystems systems, and are suitable for FAM dye detection on most modern qPCR instruments. They are optimized for use on eDNA isolates purified using Sylphiums Environmental DNA isolation kit (#SYL002), but also work on isolates purified with other eDNA isolation methods/kits.

Our assays are validated for efficiency, inhibition resistance, and compliance with NPR 7394 quality standards. When combined with appropriate sampling methods, Sylphium eDNA detection kits provide a robust and dependable solution for species detection in environmental samples.


Why choose this kit?

• High resistance to inhibiting factors. Environmental DNA (eDNA) extracts often contain various qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.
  
• Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto-fluorescent substances that will interfere with the measurements. Environmental samples need a strong fluorescence signal from the assay in order not to experience background interference.
  
• Highest possible sensitivity (1 DNA copy per reaction). Environmental water samples may contain very low amounts of target DNA.
  
• 100% specificity. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc. Not only animals from the same order (fish, amphibians, reptiles, mammals, etc.) must be taken in account during primer/probe esign, but all known DNA sequences must be checked for nonspecific binding of the primers and probe.