Sylphium offers validated tools for environmental DNA (eDNA) sampling and analysis. Our products cover the complete eDNA workflow – from water and soil collection to DNA extraction and qPCR-based detection. All products are developed and validated in-house according to NPR 7394 and CEN/TC 230 standards, ensuring reproducible and contamination-free results.
Kits include full batch documentations, validation data, and technical support. Custom-made batches and expert guidance are available on request.
eDNA sampling kits and equipment
All Sylphium sampling materials are designed for efficient and contamination-free collection of water, soil, and sediment samples. Each component is validated for use in eDNA workflows and complies with CEN/TC 230 – Water Analysis – N 1229 standards.
19 products
SYL003
SYL010-022
SYL010-045
SYL010-08
SYL010-12
SYL010-50
SYL009-022
SYL013-022
SYL013-045
SYL013-08
SYL013-12
SYL013-50
SYL006
SYL018-1
SYL018-2
SYL009-045
SYL009-08
SYL009-12
SYL009-50
Validated qPCR detection kits
Sylphium eDNA qPCR detection kits are developed for accurate and reliable detection of environmental DNA (eDNA) of target organisms. Each assay is optimized to perform consistently, also in challenging environmental matrices that contain PCR inhibitors such as humic acids.
Each kit includes the latest Sylphium eDNA qPCR hot start mix, which is optimized for environmental DNA application and is validated for accurate target detection with strong fluorescence signals and low background noise.
All kits are compatible with major qPCR platforms, including Bio-Rad, QuantStudio, and Applied Biosystems systems, and are suitable for FAM dye detection on most modern qPCR instruments. They are optimized for use on eDNA isolates purified using Sylphiums Environmental DNA isolation kit (#SYL002), but also work on isolates purified with other eDNA isolation methods/kits.
Why choose Sylphium qPCR detection kits?
- High resistance to inhibiting factors. Environmental DNA (eDNA) extracts contain often multiple qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.
- Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto-fluorescent substances that will interfere with the measurements. Environmental samples need a strong fluorescence signal from the assay in order not to experience background interference.
- Highest possible sensitivity (1 DNA copy per reaction). Environmental water samples may contain very low amounts of target DNA.
- 100% specificity. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc. Not only animals from the same order (fish, amphibians, reptiles, mammals, etc.) must be taken in account during primer/probe design, but all known DNA sequences must be checked for nonspecific binding of the primers and probe.
32 produtcs
SYL135
SYL115
SYL101
SYL128
SYL104
SYL109
SYL116
SYL159
SYL149
SYL152
SYL114
SYL103
SYL166
SYL119
SYL144
SYL150
SYL152-1
SYL106
SYL175
SYL143
SYL111
SYL139
SYL168
SYL167
SYL127
SYL131
SYL102
SYL124
SYL122
SYL165
SYL164
SYL130
DNA extraction and qPCR master mixes
Sylphium DNA extraction kits are optimized for efficient recovery of environmental DNA from water and soil samples. Each kit includes validated reagents and protocols to ensure high yield and purity, even from complex environmental matrices.
8 products
SYL1003
SYL002-20
SYL002-20-113
SYL002-50
SYL002-50-113
SYL002-100
SYL002-100-113
SYL113