Sylphium eDNA qPCR detection kits are developed for accurate and reliable detection of environmental DNA (eDNA) of target organisms. Each assay is optimized to perform consistently, also in challenging environmental matrices that contain PCR inhibitors such as humic acids.
Each kit includes the latest Sylphium eDNA qPCR hot start mix, which is optimized for environmental DNA application and is validated for accurate target detection with strong fluorescence signals and low background noise.
All kits are compatible with major qPCR platforms, including Bio-Rad, QuantStudio, and Applied Biosystems systems, and are suitable for FAM dye detection on most modern qPCR instruments. They are optimized for use on eDNA isolates purified using Sylphiums Environmental DNA isolation kit (#SYL002), but also work on isolates purified with other eDNA isolation methods/kits.
32 produtcs
SYL135
SYL115
SYL101
SYL128
SYL104
SYL109
SYL116
SYL159
SYL149
SYL152
SYL114
SYL103
SYL166
SYL119
SYL144
SYL150
SYL152-1
SYL106
SYL175
SYL143
SYL111
SYL139
SYL168
SYL167
SYL127
SYL131
SYL102
SYL124
SYL122
SYL165
SYL164
SYL130
Why choose Sylphium qPCR detection kits?
- High resistance to inhibiting factors. Environmental DNA (eDNA) extracts contain often multiple qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.
- Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto-fluorescent substances that will interfere with the measurements. Environmental samples need a strong fluorescence signal from the assay in order not to experience background interference.
- Highest possible sensitivity (1 DNA copy per reaction). Environmental water samples may contain very low amounts of target DNA.
- 100% specificity. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc. Not only animals from the same order (fish, amphibians, reptiles, mammals, etc.) must be taken in account during primer/probe design, but all known DNA sequences must be checked for nonspecific binding of the primers and probe.