Information

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eDNA sampling set – SYL009

The SYL009 eDNA sampling set is designed for efficient and clean environmental DNA (eDNA) sampling. It includes the eDNA Dual Filter capsule and valves for quick and easy sampling. The Dual Filter Capsule is engineered for on-site filtration of large volumes of environmental water, minimizing cross-contamination risks. Featuring an optimal pore size of 0.8 micron for effective eDNA filtration, the capsule prevents rapid clogging with its dual-membrane design, offering a substantial filtering surface area. The complete eDNA sampling sets are also available with filter membranes with different pore sizes.

After use, the eDNA Dual Filter Capsule can be securely closed with luer lock caps. It is suitable for in-capsule lysing and preservation, with storage at room temperature. During eDNA extraction, solid residues remain on the membrane, ensuring smooth downstream processing. The eDNA Sampling Set meets all requirements outlined in CEN/TC 230 – Water Analysis – N 1229.


eDNA sampling set features

• Ease of use: simple (sub)sampling without pooling
  
• High yield: large membrane surface and optimal pore size for efficient eDNA filtration
  
• No contamination: enclosed filter membranes and in-capsule conservation
  
• Clean eDNA isolates: in-capsule removal of PCR inhibitors during filtration and extraction

Superior performance: large volumes and high eDNA yields

Compared to traditional filtering methods in eDNA studies, the eDNA Dual Filter Capsule can filter nearly 10 times more water and eDNA than a filter funnel and 20 times more than a Sterivex filter. This results in significantly higher eDNA yields.


Product specifications

• Filter unit type: closed capsule
  
• Effective filter area: 69 squared centimetres (dual filter system)
  
• Filter membrane: polyethylene sulfone (PES)
  
• Pore sizes: 0.22, 0.45, 0.8, 1.2, and 5 micron
  
• Maximum inlet pressure: 3 Bar (45 psi)*
  
• Inlet and outlet fittings: Luer-lock female
  
• Cartrigde material: non-toxic acrylonitrile Butadiene styrene (ABS)
  
• Sterilization method: gamma irradiation (on request)

*Note: The filter capsules are designed for vacuum filtration, meaning that water should be drawn into the filter rather than forced through it. When using a pump to push water through the filter membranes, ensure that the maximum inlet pressure is not exceeded. For instance, a rotary vane pump can easily generate high pressures, which may cause leakage in both the inner and outer structures of the capsule.


Set contents (20 sets per box)

• eDNA Dual Filter capsule with valve connector
  
• 60 mL syringe
  
• 3.5 ml sample preservation buffer* in 5 ml syringe (includes xenobiotic internal positive control)
  
• Luer-lock male caps (2x)
  
• Storage bag

*Extensive stability tests have shown that DNA concentration in the preservation solution remains stable for at least two years. These tests, which included an internal positive standard, verified the integrity and consistency of the eDNA concentration. Additionally, potential contaminants from the water matrix were monitored, showing no significant effects on the buffer its efficacy after three months, ensuring effective preservation of eDNA over long periods.