Information

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The environmental DNA qPCR hot start mix offers outstanding performance for sensitive, robust and precise probe-based qPCR detection and quantitation of target eDNA extracted from environmental samples. eDNA qPCR hot start mix is highly resistant to inhibiting factors, like humic acids in environmental samples.


Kit contents

• 2x 2500 ul eTaq qPCR mix (2x)
  
• 2x 200 ul eTaq DNA polymerase

The eDNA qPCR Mix has:

• High resistance to inhibiting factors. Environmental DNA (eDNA) extracts contain often multiple qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.
  
• Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto-fluorescent substances that will interfere with the measurements. Environmental samples need a strong fluorescence signal from the assay in order not to experience background interference.
  
• Highest possible sensitivity (1 DNA copy per reaction). Environmental water samples may contain very low amounts of target DNA.

Reference:

Uchii, K., Doi, H., Okahashi, T., Katano, I., Yamanaka, H., Sakata, M. K., et al. (2019). Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA. DNA 1, 359-367.