Information

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All Sylphium sample sets include an Internal Positive Control (IPC) within the preservation solution, located in the small syringe of the sampling set (SYL009). The IPC serves as an efficiency check for the DNA isolation procedure, ensures the quality and purity of the isolated eDNA, and helps prevent false negative results. This internal control consists of a small piece of synthetic xenobiotic DNA. The selected positive control DNA sequence is not found in the aquatic environment and does not interfere with the detection of target organisms. Additionally, the IPC Quantification Kit can be ordered separately or together with the eDNA Isolation Kit. The IPC quantification kit can be ordered seperately or together with the eDNA isolation kit.

This kit has been developed and optimized for use on eDNA isolates purified using Sylphiums Environmental DNA isolation kit (#SYL002), but also works on isolates purified with other eDNA isolation methods/kits.


Kit contents IPC quantification kit:

• Positive control
  
• Primer/Probe mix for detection of the Internal Positive Control (FAM dye)
  
• eDNA qPCR Master Mix, containing:
  
  • eTaq DNA polymerase
    
  • eTaq qPCR mix
    
  • PCR-grade water

Sufficient for 200 reactions of 25 ul each.